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Figure 4 Increases in (–)isoprenaline-stimulated cAMP-levels (10–12 to 10–5 mol/liter) upon incubation of CHW-beta1 cells with a fixed concentration of rabbit (70 nmol/liter) or human anti-beta1-ECII (2 µmol/liter; patient-subgroups A and B). Filled circles or diamonds correspond to the values obtained for rabbit anti-beta1-C or IgG-preparations from healthy subjects, respectively. Error bars indicate the mean ± SD of three experiments. Significance between control values (filled symbols) and those obtained in the presence of anti-beta1-ECII (open symbols) was analyzed by the Scheffé F test. All the values obtained for patient anti-beta1-ECII (subgroups A/B) differed significantly (p < 0.05) from control values in the presence of low (10–12 to 10–10 mol/liter) or high (10–9 to 10–5 mol/liter) isoprenaline concentrations, except those from patient subgroup B at 10–10 mol/liter isoprenaline (p = 0.15, not significant).