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Figure 2 Decrease in ligand-binding to human beta₁-AR expressed in Sf9 cells (a) in the presence of constant 3 nmol/liter [³H]CGP 12177 and the indicated concentrations of anti-beta₁-AR, or (b) in the presence of a fixed concentration of the different anti-beta₁-AR (mouse, 7 nmol/liter; rabbit, 70 nmol/liter; human: 2 µmol/liter) and various concentrations of [³H]CGP 12177 (3 x 10^–11 to 5 x 10^–9 mol/liter). Error bars indicate the mean ± SD of three experiments. B_max = total ligand binding capacity; K_D = receptor-ligand dissociation constant.