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Figure 4 17 Estradiol (E2) does not prevent hypoxia-reoxygenation–induced Ca²⁺ loading in female cardiomyocytes. Epifluorescent digital images of Fluo-3AM loaded cardiac cells from female hearts pretreated with 10 nmol/liter E2 prior to and following hypoxia-reoxygenation (H/R). Horizontal bar corresponds to 30 µm. (A1) Time-course of Fluo-3AM fluorescence in cell presented in A. AU: arbitrary units. (B) Average concentration of intracellular Ca²⁺ at rest and following hypoxia-reoxygenation. Bars represent mean ± SEM (n = 6).