Click on image to view larger version.
Figure 3 Tamoxifen blocks the protective action of E2. Epifluorescent digital images of Fluo-3AM loaded cardiac cells from female hearts, pretreated with 10 nmol/liter E2 plus 1 µmol/liter tamoxifen, prior to and following hypoxia-reoxygenation (H/R). Horizontal bar corresponds to 30 µm. (A1) Time-course of averaged Fluo-3 fluorescence in cell presented in A. AU = arbitrary units. (B) Average concentration of intracellular Ca2+ at rest and following hypoxia-reoxygenation. Bars represent mean ± SEM (n = 5).