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Figure 2 The 17ß-estradiol (E2) prevents decreases hypoxia-reoxygenation–induced Ca²⁺ loading in female, but not male, cardiomyocytes. Epifluorescent digital images of Fluo-3AM loaded, and 10 nmol/liter pretreated cardiac cells from female (A) and male (B) hearts prior to and following hypoxia-reoxygenation (H/R). Horizontal bar corresponds to 30 µm. Time-course of averaged Fluo-3 fluorescence in cells presented in A (A1) and B (B1). AU: arbitrary units. (C) Average concentration of intracellular Ca²⁺ at rest, and following hypoxia-reoxygenation. Bars represent mean ± SEM (n = 6–7).