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Figure 3 (A) Negative TUNEL reactivity, (B) positive TUNEL DNAse 1 treated control, (C) TUNEL positive reactivity (green) in a DCM sample, (D) propidium iodide (PI) staining (red) of section C, (E) double exposure of section C and D showing a myocyte that is positive for TUNEL and for PI (orange) and (F) alpha-sarcomeric actin staining of a serial section of C. DCM = dilated cardiomyopathy; TUNEL = terminal deoxynucleotidyl transferase [TdT]-mediated dUTP nick end labelling.