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Figure 5 Mitochondrial enzyme activity in cardiac myocytes. Myocytes were incubated in the presence of 10 ng/ml IL-1ß (IL), with or without 3 mmol/liter N^G-monomethyl-L-arginine (L-NMMA), and 10^–4 mol/liter or 10^–3 mol/liter sodium nitroprusside (SNP). The activity of the indicated mitochondrial enzymes was assayed as described in Methods. Control preparations received no additives. All activities are in units of µmol/min/mg protein. p < 0.01; p < 0.0001 vs. control (n = 6).