Click on image to view larger version.

Figure 5 Electrophoretic mobility shift assays demonstrating the effects of NO donor, spermine/NO, on NF-kappaB DNA binding activity (representative of three similar experiments). Supershifted immunoreactive band using antibodies against the p50 and p65 subunits of NF-kappaB are indicated by the arrows. NF-kappaB = nuclear factor kappa B; NO = nitric oxide; Spermine = the carrier vehicle for the NO donor.