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Figure 4 Absence of regulation of G_s and G_i proteins in pulmonary membrane preparations from rats with CHF (hatched bars) and controls (open bars) (n = 3). The G protein subunits were detected by specific antibodies and visualized by a chemoluminescence technique (see Methods). Values derived from the densitometric analysis (AUC, area under the curve) are shown as percent of controls. Insets depict respective lanes from representative experiments running at 40 kDa.