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Figure 6 Identification of estrogen receptor transcript by reverse transcriptase polymerase chain reaction (RT-PCR). Figure shows RT-PCR products in adult rat female and male myocytes, and in left ventricular (LV) tissue from female and male LV hypertrophy (LVH) hearts. The sequence of the major 741-bp product was homologous to uterine estrogen receptor. No PCR products were obtained from negative control samples in the absence of reverse transcriptase (lanes 2, 4, 6, 8).