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Figure 6 Expression of 5'-adenosine monophosphate–activated protein kinase (AMPK) (alpha2 subfraction) in left ventricular myocardium of fetal, newborn, 3-week old control and volume-overloaded animals. Inset shows a representative Western transfer, probed with ovine raised anti-alpha2AMPK antibodies demonstrating the 63-kDa region comprising cardiac AMPK. Densitometric analysis is expressed as a percentage of expression in fetal LV myocardium with the mean in each group determined from 6 to 14 individual hearts each analyzed once. Abbreviations as in Figure 5. *p < 0.05, newborn and control vs. fetal; {dagger}p < 0.05, hypertrophy vs control (analysis of variance).