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Figure 5 Quantification of iNOS mRNA in the hearts using RT-PCR. A: Total RNA was extracted from the hearts in the pimobendan 1 mg/kg group and the control group. Each PCR product was separated by electrophoresis on a 5% polyacrylamide gel. The gels were dried and autoradiographed. nh-DNA = nonhomologous DNA. B: The log of the ratio of iNOS or G3PDH target radioactivity to the nh-DNA fragment radioactivity was plotted against the log of the number of the nh-DNA fragment molecules added to each PCR reaction. The number of iNOS or G3PDH cDNA molecules was obtained from the point where log (iNOS or G3PDH/the nh-DNA fragment) was equal to zero. C: The number of iNOS or G3PDH cDNA was expressed as attomoles/µg of total RNA. The iNOS gene expression in the pimobendan 1 mg/kg group was 74% lower than that of the control group. * = p < 0.01 versus control.