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Figure 3 Result of iNOS mRNA detection by RT-PCR in left ventricular myocardial preparations from two nonfailing hearts incubated for 12 h either in normal tyrode solution (control) or in tyrode plus lipopolysaccharides (1 µg/ml). Amplification products of iNOS-PCR were loaded on the left side, those of GAPDH-PCR on the right side of the gel. Notice that only in two of six nonfailing hearts shown in this figure, iNOS mRNA was not already detectable in control samples, and that iNOS mRNA was detectable in all samples from failing hearts (n = 6).