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Figure 3 Immunohistochemistry for MMP-9 (A) and macrophages (B) in high power fields (x40, reduced by 65%). Matrix metalloproteinase-9 positive cells (arrowheads) are spread homogeneously throughout the infarcted region, and macrophages are shown infiltrating the border region next to myocardial cells (arrow). Scale bars in (A) and (B) represent 55 µm. Double immunolabeling (C) for macrophages (blue-stained cells) and MMP-9 (red-stained cells) in high power fields (x100, reduced by 65%) demonstrates double-stained cells (purple cells, arrow). Scale bar in (C) represents 12 µm.