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Figure 3 Isolated neutrophil CD11b expression. Neutrophils isolated from peripheral blood of healthy adult volunteers were incubated with 3.5 µg/ml chimeric 7E3 Fab at 37°C for 30 min and then stimulated with fMLP (10 nmol/liter). There was no difference in CD11b expression with or without chimeric 7E3, either before or after stimulation with fMLP (paired t test, n = 5). Two phycoerythrin-conjugated monoclonal antibodies were studied to confirm this finding (Leu-15, Becton Dickinson Immunocytometry Systems, San Jose, California; 2LPM19c, DAKOpatts, Denmark).