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Figure 7 Atorvastatin modulation of NF-B activity and MCP-1 expression in VSMC. Vascular smooth muscle cells were growth-arrested by serum deprivation for 48 h and then preincubated with 10^–7 mol/L atorvastatin for 1 h before the addition of 100 U/mL of TNF. Cells were then cultured for 6 h to study MCP-1 expression by Northern blot and for 1 h to determine NF-B activity by EMSA. G3PDH mRNA expression was used to normalize the MCP-1 expression. A representative blot of three experiments done is shown.