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Figure 5 NF-B activity in the lesions. NF-B activity was determined by Southwestern histochemistry in untreated (A), atorvastatin treated (B) and control (C) animals. The binding of the labeled probe is specific since no staining is found with the mutant oligonucleotide (D) (magnification x400). A detail of the localization of the NF-B activity by double staining with anti–macrophage antibody is shown in E and with anti-alpha-actin in F (magnification x1000). The arrows indicate the presence of a nuclei stained for NF-B (blue) and surrounded by a cytoplasm (brown) stained with the antibody specific for macrophages (E) and for VSMC (F).