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Figure 3 Representative Northern blot showing mRNA levels of ANP, BNP and beta-2-microglobulin (BMG). Two µg and 5 µg of total RNA per lane were hybridized with ³²P-labeled ANP and BNP cDNA probes, respectively, and they were rehybridized with ³²P-labeled BMG cDNA probes. Mean right atrial pressure (mRAP) of each patient was indicated above the lane number (lane 1 through 5). Lane 1, patient 15 in Table 1; lane 2, patient 13; lane 3, patient 8; lane 4, patient 3; lane 5, patient 1, respectively.