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Figure 1 RNase protection analysis with antisense-RNA probes specific for human NOS II and NOS III. In lane 1 the unhybridized NOS II and NOS III antisense-probes are shown. Lanes 2 to 14 show hybridizations of the respective RNA samples with both antisense-probes: 2, total RNA from yeast (negative control); 3 to 10, increasing amounts of NOS II and NOS III sense-RNA (3 to 4: 0.5 pg, 5 to 6: 2.5 pg, 7 to 8: 5 pg, 9: 10 pg, 10: 20 pg); 11 to 12, total RNA from NF human hearts; 13 to 14, total RNA from failing human hearts.