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Figure 4 Immunoblot against SERC2a (110 kD), calsequestrin (63 kD), pentameric phospholamban (25 kD), and monomeric phospholamban (25 kD). Samples were each immunoprecipitated with anti-SERC2a, calsequestrin, or phospholamban selective antibodies, separated by SDS-PAGE on a 4–20% acrylamide gel, and then visualized by autoradiography. With increasing age, protein concentration of SERC2a and pentameric phospholamban decrease with age, while calsequestrin and monomeric phospholamban remain relatively constant.